Single Nucleotide Polymorphism (SNP) Discovery and Association Study of Flowering Times, Crude Fat and Fatty Acid Composition in Rapeseed (Brassica napus L.) Mutant Lines Using Genotyping-by-Sequencing (GBS)

Rapeseed is the most important oil crop used in food and biodiesel industries. In this study, based on single nucleotide polymorphism (SNP) identified from genotyping-by-sequencing (GBS), an association study of flowering time, crude fat fatty acid contents were investigated 46 rapeseed mutant lines derived gamma rays. A total 623,026,394 clean data reads generated with 6.6 million average. set 37,721 filtered SNPs was to perform gene ontology phylogenetic analysis. Hierarchical cluster analysis gave eight groups time compositions. Gene ontological showed that many genes displaying are involved cellular processes, anatomy, binding. 40 significantly associated (1 SNP), content (2 SNPs), (37 SNPs). 21 annotated SNPs; among them, nine enriched reproductive such as embryonic development, fruit seed development. This demonstrated efficient tools for screening it provides a basis improving qualities rapeseed.